SEQanswers - Sequencing Technologies/Companies https://www.seqanswers.com/ Platform specific questions, news, discussions en Sat, 24 Oct 2026 06:44:53 GMT vBulletin 60 https://www.seqanswers.com/images/misc/rss.png SEQanswers - Sequencing Technologies/Companies https://www.seqanswers.com/ weird Q30 drop in the recent nextseq run https://www.seqanswers.com/forum/sequencing-technologies-companies/illumina-solexa/327720-weird-q30-drop-in-the-recent-nextseq-run Fri, 23 Oct 2026 15:53:24 GMT This is the first time I noticed the Q30 drop in the middle of the cycles, but goes up at the end of the run. Does anyone have experience with this?... Thanks in advance!

The library is bulk RNA libary. So the diversity should not be the problem.]]> Illumina (Solexa) allenyang2003 https://www.seqanswers.com/forum/sequencing-technologies-companies/illumina-solexa/327720-weird-q30-drop-in-the-recent-nextseq-run