Min score function: 0.0 + -0.2 * x Mismatch (mm) penalty: -6 Gap open penalty (read or reference): -5 Gap extend penalty (read or reference): -3 x Min_sc AS=0 AS=-6 AS=-8 AS=-11 AS=-12 AS=-14 AS=-18 AS=-24 25 -5.00 42 0 0 0 0 0 0 0 26 -5.20 42 0 0 0 0 0 0 0 27 -5.40 42 0 0 0 0 0 0 0 28 -5.60 42 0 0 0 0 0 0 0 29 -5.80 42 0 0 0 0 0 0 0 30 -6.00 42 0 0 0 0 0 0 0 31 -6.20 42 0 0 0 0 0 0 0 32 -6.40 42 0 0 0 0 0 0 0 33 -6.60 42 0 0 0 0 0 0 0 34 -6.80 42 0 0 0 0 0 0 0 35 -7.00 42 0 0 0 0 0 0 0 36 -7.20 42 0 0 0 0 0 0 0 37 -7.40 42 0 0 0 0 0 0 0 38 -7.60 42 0 0 0 0 0 0 0 39 -7.80 42 0 0 0 0 0 0 0 40 -8.00 42 0 0 0 0 0 0 0 41 -8.20 42 0 0 0 0 0 0 0 42 -8.40 42 0 0 0 0 0 0 0 43 -8.60 42 3 0 0 0 0 0 0 44 -8.80 42 3 0 0 0 0 0 0 45 -9.00 42 3 0 0 0 0 0 0 46 -9.20 42 3 0 0 0 0 0 0 47 -9.40 42 3 0 0 0 0 0 0 48 -9.60 42 3 0 0 0 0 0 0 49 -9.80 42 3 0 0 0 0 0 0 50 -10.00 42 8 0 0 0 0 0 0 55 -11.00 42 8 0 0 0 0 0 0 60 -12.00 42 23 3 0 0 0 0 0 65 -13.00 42 23 3 0 0 0 0 0 70 -14.00 42 23 8 0 0 0 0 0 75 -15.00 42 24 8 0 0 0 0 0 80 -16.00 42 24 23 3 0 0 0 0 85 -17.00 42 24 23 3 0 0 0 0 90 -18.00 42 24 23 3 3 0 0 0 95 -19.00 42 24 23 8 3 0 0 0 100 -20.00 42 40 24 8 8 3 0 0 120 -24.00 42 40 24 23 23 8 0 0 140 -28.00 42 40 40 24 23 23 3 0 160 -32.00 42 42 40 24 24 23 8 0 180 -36.00 42 42 40 24 24 24 23 3 200 -40.00 42 42 42 40 40 24 23 8 Min score function: -0.6 + -0.6 * x Mismatch (mm) penalty: -6 Gap open penalty (read or reference): -5 Gap extend penalty (read or reference): -3 x Min_sc AS=0 AS=-6 AS=-8 AS=-11 AS=-12 AS=-14 AS=-18 AS=-24 25 -15.60 42 24 8 0 0 0 0 0 26 -16.20 42 24 23 3 0 0 0 0 27 -16.80 42 24 23 3 0 0 0 0 28 -17.40 42 24 23 3 3 0 0 0 29 -18.00 42 24 23 3 3 0 0 0 30 -18.60 42 24 23 8 3 0 0 0 31 -19.20 42 24 23 8 3 0 0 0 32 -19.80 42 24 23 8 3 0 0 0 33 -20.40 42 40 24 8 8 3 0 0 34 -21.00 42 40 24 8 8 3 0 0 35 -21.60 42 40 24 8 8 3 0 0 36 -22.20 42 40 24 23 8 3 0 0 37 -22.80 42 40 24 23 8 3 0 0 38 -23.40 42 40 24 23 8 8 0 0 39 -24.00 42 40 24 23 23 8 0 0 40 -24.60 42 40 24 23 23 8 0 0 41 -25.20 42 40 24 23 23 8 0 0 42 -25.80 42 40 24 23 23 8 3 0 43 -26.40 42 40 24 23 23 8 3 0 44 -27.00 42 40 40 23 23 8 3 0 45 -27.60 42 40 40 24 23 8 3 0 46 -28.20 42 40 40 24 23 23 3 0 47 -28.80 42 40 40 24 23 23 3 0 48 -29.40 42 40 40 24 23 23 3 0 49 -30.00 42 42 40 24 24 23 8 0 50 -30.60 42 42 40 24 24 23 8 0 55 -33.60 42 42 40 24 24 23 8 0 60 -36.60 42 42 40 24 24 24 23 3 65 -39.60 42 42 40 40 24 24 23 3 70 -42.60 42 42 42 40 40 24 23 8 75 -45.60 42 42 42 40 40 24 24 8 80 -48.60 42 42 42 40 40 40 24 23 85 -51.60 42 42 42 40 40 40 24 23 90 -54.60 42 42 42 40 40 40 24 23 95 -57.60 42 42 42 42 40 40 24 23 100 -60.60 42 42 42 42 42 40 40 24 120 -72.60 42 42 42 42 42 42 40 24 140 -84.60 42 42 42 42 42 42 40 40 160 -96.60 42 42 42 42 42 42 42 40 180 -108.60 42 42 42 42 42 42 42 40 200 -120.60 42 42 42 42 42 42 42 42 Min score function: 0.0 + -0.4 * x Mismatch (mm) penalty: -6 Gap open penalty (read or reference): -5 Gap extend penalty (read or reference): -3 x Min_sc AS=0 AS=-6 AS=-8 AS=-11 AS=-12 AS=-14 AS=-18 AS=-24 25 -10.00 42 8 0 0 0 0 0 0 26 -10.40 42 8 0 0 0 0 0 0 27 -10.80 42 8 0 0 0 0 0 0 28 -11.20 42 8 0 0 0 0 0 0 29 -11.60 42 8 3 0 0 0 0 0 30 -12.00 42 23 3 0 0 0 0 0 31 -12.40 42 23 3 0 0 0 0 0 32 -12.80 42 23 3 0 0 0 0 0 33 -13.20 42 23 3 0 0 0 0 0 34 -13.60 42 23 8 0 0 0 0 0 35 -14.00 42 23 8 0 0 0 0 0 36 -14.40 42 23 8 0 0 0 0 0 37 -14.80 42 23 8 0 0 0 0 0 38 -15.20 42 24 8 0 0 0 0 0 39 -15.60 42 24 8 0 0 0 0 0 40 -16.00 42 24 23 3 0 0 0 0 41 -16.40 42 24 23 3 0 0 0 0 42 -16.80 42 24 23 3 0 0 0 0 43 -17.20 42 24 23 3 3 0 0 0 44 -17.60 42 24 23 3 3 0 0 0 45 -18.00 42 24 23 3 3 0 0 0 46 -18.40 42 24 23 8 3 0 0 0 47 -18.80 42 24 23 8 3 0 0 0 48 -19.20 42 24 23 8 3 0 0 0 49 -19.60 42 24 23 8 3 0 0 0 50 -20.00 42 40 24 8 8 3 0 0 55 -22.00 42 40 24 23 8 3 0 0 60 -24.00 42 40 24 23 23 8 0 0 65 -26.00 42 40 24 23 23 8 3 0 70 -28.00 42 40 40 24 23 23 3 0 75 -30.00 42 42 40 24 24 23 8 0 80 -32.00 42 42 40 24 24 23 8 0 85 -34.00 42 42 40 24 24 23 8 0 90 -36.00 42 42 40 24 24 24 23 3 95 -38.00 42 42 40 40 24 24 23 3 100 -40.00 42 42 42 40 40 24 23 8 120 -48.00 42 42 42 40 40 40 24 23 140 -56.00 42 42 42 42 40 40 24 23 160 -64.00 42 42 42 42 42 40 40 24 180 -72.00 42 42 42 42 42 42 40 24 200 -80.00 42 42 42 42 42 42 40 40