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  • SNPsaurus
    replied
    For isolating DNA that has high divergence, pulldown by hybridization is usually better. You can order pulldown baits from any oligo supplier, such as IDT https://www.idtdna.com/pages/product...target-capture, or MYcroarray for larger regions.

    Leave a comment:


  • riv
    replied
    Hi! I am not sure if I got your question right, but I have these ideas:

    These genes that you are interested on maybe have some region that is more conserved and therefore it can be amplified with PCR. Even if you get a tiny part, you can do RACE PCR after that.

    If you only have the sequences in 2 species and you want to check which parts should be more conserved, you can look for those genes in another taxa and make some multiple alignment.

    Anyway, I know that optimizing PCR can be really annoying. If somebody has another idea, I´m interested too.

    Leave a comment:


  • flobpf
    started a topic Method for targeted sequencing

    Method for targeted sequencing

    Hi,

    My goal is to obtain the sequence of a gene from several species. I know the sequence only in 2 species separated by ~30mya. Designing optimal primers is not an option, since if the PCR reaction doesn't work in one species, I wouldn't know whether its because the primers failed or the gene is absent. Southern is too cumbersome, in my opinion. Wonder if there is another way to do my job? I checked for commercial kits but most kits for targeted sequencing are in humans and I'm working in a non-model plant lineage.

    I want to stress that I only want to get only selected loci (5 genes in my case)...

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    Multiomics Techniques Advancing Disease Research
    by seqadmin


    New and advanced multiomics tools and technologies have opened new avenues of research and markedly enhanced various disciplines such as disease research and precision medicine1. The practice of merging diverse data from various ‘omes increasingly provides a more holistic understanding of biological systems. As Maddison Masaeli, Co-Founder and CEO at Deepcell, aptly noted, “You can't explain biology in its complex form with one modality.”

    A major leap in the field has
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