Hi,
I am planning to generate miRNA data using an Illumina GAII and I am a beginner in the miRNA world. Can you please give some detail of your experimental design? My plan is to prepare 10 different libraries each one tagged with the Illumina index and run them together in the same lanes og the GA. My questions are:
1) is a sequencing kit for the sequencing of 36 bp enough to cover the entire miRNAs length or, because of adapters and primers, I need the single end 75 bp reads (2 sequencing kits)?
2) considering that I multiplex 10 different species in each lane, how many lanes I should use to have a good representation of miRNAs for each species? The species I am working have a genome size of about 1Gb.
I appreciate your help.
Thanks
Seqanswers Leaderboard Ad
Collapse
Announcement
Collapse
No announcement yet.
X
-
You can try the DSAP package
http://dsap.cgu.edu.tw.
DSAP use the non-coding RNA database PFam to remove all small RNA populatopms other than microRNA.
Leave a comment:
-
Small RNA data analysis
I am analyzing a small RNA data set that we generate from Illumina GAII.
This organism doesn't have a sequenced genome. How can I get rid of rRNA, tRNA, snRNA, and snoRNA, as well as those containing the polyA tail?
Latest Articles
Collapse
-
by seqadmin
Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...-
Channel: Articles
03-22-2024, 06:39 AM -
-
by seqadmin
The field of conservation genomics centers on applying genomics technologies in support of conservation efforts and the preservation of biodiversity. This article features interviews with two researchers who showcase their innovative work and highlight the current state and future of conservation genomics.
Avian Conservation
Matthew DeSaix, a recent doctoral graduate from Kristen Ruegg’s lab at The University of Colorado, shared that most of his research...-
Channel: Articles
03-08-2024, 10:41 AM -
ad_right_rmr
Collapse
News
Collapse
Topics | Statistics | Last Post | ||
---|---|---|---|---|
Started by seqadmin, Yesterday, 06:37 PM
|
0 responses
8 views
0 likes
|
Last Post
by seqadmin
Yesterday, 06:37 PM
|
||
Started by seqadmin, Yesterday, 06:07 PM
|
0 responses
8 views
0 likes
|
Last Post
by seqadmin
Yesterday, 06:07 PM
|
||
Started by seqadmin, 03-22-2024, 10:03 AM
|
0 responses
49 views
0 likes
|
Last Post
by seqadmin
03-22-2024, 10:03 AM
|
||
Started by seqadmin, 03-21-2024, 07:32 AM
|
0 responses
66 views
0 likes
|
Last Post
by seqadmin
03-21-2024, 07:32 AM
|
Leave a comment: