Dear All,
Does anyone of you have experience with MeDIP-seq? We are currently trying to do this sequencing using Illumina/ Solexa technology, but we couldn´t get enough reads.
Normally we get around 15 million reads, but for our sample the maximum that we could get is 700 000.
Do we need some special parameters during the cluster generations for the MeDIP sample? for example adding betain or increasing the T° of the denaturation before the first sequencing cycle?
I will be very grateful if someone share his experience with us.
Thanks in advance
Regards
Does anyone of you have experience with MeDIP-seq? We are currently trying to do this sequencing using Illumina/ Solexa technology, but we couldn´t get enough reads.
Normally we get around 15 million reads, but for our sample the maximum that we could get is 700 000.
Do we need some special parameters during the cluster generations for the MeDIP sample? for example adding betain or increasing the T° of the denaturation before the first sequencing cycle?
I will be very grateful if someone share his experience with us.
Thanks in advance
Regards
Comment