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Who uses a MiSeq for 16S data?

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  • Who uses a MiSeq for 16S data?

    Hi Everyone,

    I'm interested to hear about anyone's experiences with 16S sequencing on the MiSeq. I'm aware of the papers by Caporaso, Illumina's tech notes, methods for 'hard-coding' matrix and phasing values, etc. What I'm interested in is actual experiences: What methods are you using for primer design and indexing? How many indexes are you running per lane? What kind of quality are you getting? What kind yield? Etc.

    Cheers!
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