Header Leaderboard Ad

Collapse

Degseq Question

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Degseq Question

    Hello All,

    I am new here in this awesome forum, new to the topics you guys talking about as well. I've read about Degseq and I'm trying to work on it. So, I have installed the R and Degseq and now as a beginner I am working on the example provided in their manual with sample data that comes with Degseq. they started the example with the following command, which went fine:
    > library(DEGseq)
    Then this:
    >geneExpFile <- system.file("extdata", "GeneExpExample5000.txt",+ package = "DEGseq")

    This one keeps returns this error msg:

    Error: unexpected '=' in "geneExpFile <- system.file("extdata", "GeneExpExample5000.txt",+ package ="


    Any Help is appreciated

  • #2
    Originally posted by Amative View Post
    Then this:
    >geneExpFile <- system.file("extdata", "GeneExpExample5000.txt",+ package = "DEGseq")
    Hey there,

    I had a similar problem when I started with R and DEGseq.
    There is a problem where the R syntax and the DEGseq tutorial collide.

    So in R on the console every line begins with a ">" However you never input this character, it's just there. If you then type a multilin statement, R shows by a "+" that this line is just continuing the line above.

    Therefore if you copy and paste the examples, you have to remove the > and + characters, as in:
    geneExpFile <- system.file("extdata", "GeneExpExample5000.txt", package = "DEGseq")

    Also "r getting started" is a good google query to get started with R.

    Best,
    Simon

    Comment


    • #3
      Thanks Simon,

      geneExpFile <- system.file("extdata", "GeneExpExample5000.txt",+ package = "DEGseq")

      should be

      geneExpFile <- system.file("extdata", "GeneExpExample5000.txt", package = "DEGseq")

      SO, I just removed the + sign )

      Comment

      Latest Articles

      Collapse

      • seqadmin
        Improved Targeted Sequencing: A Comprehensive Guide to Amplicon Sequencing
        by seqadmin



        Amplicon sequencing is a targeted approach that allows researchers to investigate specific regions of the genome. This technique is routinely used in applications such as variant identification, clinical research, and infectious disease surveillance. The amplicon sequencing process begins by designing primers that flank the regions of interest. The DNA sequences are then amplified through PCR (typically multiplex PCR) to produce amplicons complementary to the targets. RNA targets...
        Yesterday, 01:49 PM
      • seqadmin
        Targeted Sequencing: Choosing Between Hybridization Capture and Amplicon Sequencing
        by seqadmin




        Targeted sequencing is an effective way to sequence and analyze specific genomic regions of interest. This method enables researchers to focus their efforts on their desired targets, as opposed to other methods like whole genome sequencing that involve the sequencing of total DNA. Utilizing targeted sequencing is an attractive option for many researchers because it is often faster, more cost-effective, and only generates applicable data. While there are many approaches...
        03-10-2023, 05:31 AM
      • seqadmin
        Expert Advice on Automating Your Library Preparations
        by seqadmin



        Using automation to prepare sequencing libraries isn’t a new concept, and most researchers are aware that there are numerous benefits to automating this process. However, many labs are still hesitant to switch to automation and often believe that it’s not suitable for their lab. To combat these concerns, we’ll cover some of the key advantages, review the most important considerations, and get real-world advice from automation experts to remove any lingering anxieties....
        02-21-2023, 02:14 PM

      ad_right_rmr

      Collapse

      News

      Collapse

      Topics Statistics Last Post
      Started by seqadmin, 03-17-2023, 12:32 PM
      0 responses
      14 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 03-15-2023, 12:42 PM
      0 responses
      18 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 03-09-2023, 10:17 AM
      0 responses
      68 views
      1 like
      Last Post seqadmin  
      Started by seqadmin, 03-03-2023, 12:03 PM
      0 responses
      64 views
      0 likes
      Last Post seqadmin  
      Working...
      X