RNAClean XP

[wschneider]

Does anyone know whether RNAClean XP efficiently captures small (< 100bp) RNA/cDNA?

The website states under Key Features: Purification of small and large nucleic acid products, but it seems that most products based on bead technology do not efficiently capture products smaller than 100bp.

I am interested in preparing samples for RNA-seq that include all species of RNA including mRNA as well as miRNA and everything in between. Most of the protocols I have come across focus on one population or the other where miRNAs are generally isolated by gel electrophoresis and "total RNA" samples exclude RNA that is smaller than 100bp.

[Jon_Keats]

Not sure about that product but do you have a custom library prep? Most commercial methods do not support both miRNA and mRNA analysis.

[wschneider]

Thanks for the reply. I don't have any custom library prep in mind so that would explain why it's so difficult to find any examples in the literature.

What step(s) is incompatible between the sample preps? Does it have to do with using random hexamers on a small RNA to generate a cDNA copy? I suppose if I wanted both large and small RNAs the best approach would be to fractionate the sample and process them separately.