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How can I calculate RPM value for a gene to validate my results ?
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Any one please help me with this.
How should I calculate RPM values for a gene list ? -
Maybe if you were a bit more specific, you would get an answer.
"RPM" means "reads per million", i.e., you count how many reads map to your gene, divide by the total number of aligned reads and multiply by one million. So, I guess, (assuming that you know how to carry out a division) that you want to know how to count how many reads map onto a gene. My htseq-count script is one option, BEDtools or easyRnaSeq are others.Comment
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