Small RNA sequencing depth comparison to array or qPCR - SEQanswers

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seqfan2011

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Join Date: May 2012

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Small RNA sequencing depth comparison to array or qPCR

05-03-2012, 08:08 AM

Hi,

Has anyone came across small RNA-seq comparison study with array or qPCR? Such as how many millions reads would be needed to reach the senstivity of qPCR for detection of small RNA at CT value of 30 from 5-10ng input....

Thanks
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