I'd like to perform a targeted RNA-seq experiment to not only identify splice variants but also measure gene expression levels of the normal and abnormal variants I capture.
Does anyone have any suggestions or direct me to some publications that can provide some guidelines as to the number of housekeeping transcripts I should include in my capture design such that my bioinformatics group can provide me with expression levels of the targeted transcripts/novel splice variants I want to capture?
From my qPCR experience I was thinking that at least 3 would be needed but figured I'd ask the broader community to see if there was any consensus.
-
The COVID-19 pandemic highlighted the need for proactive pathogen surveillance systems. As ongoing threats like avian influenza and newly emerging infections continue to pose risks, researchers are working to improve how quickly and accurately pathogens can be identified and tracked. In a recent SEQanswers webinar, two experts discussed how next-generation sequencing (NGS) and machine learning are shaping efforts to monitor viral variation and trace the origins of infectious...03-24-2025, 11:48 AM -
This year’s Advances in Genome Biology and Technology (AGBT) General Meeting commemorated the 25th anniversary of the event at its original venue on Marco Island, Florida. While this year’s event didn’t include high-profile musical performances, the industry announcements and cutting-edge research still drew the attention of leading scientists.
The Headliner
The biggest announcement was Roche stepping back into the sequencing platform market. In the years since...03-03-2025, 01:39 PM