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  • dpryan
    replied
    Cross-posted on biostars, where I already answered.

    Leave a comment:


  • onthefly
    started a topic DEXSeq error while creating ExonCountSet object

    DEXSeq error while creating ExonCountSet object

    Hi

    I am using DEXSeq for my analysis.

    However, I am facing error while making the ExonCountSet object for further processing.

    Specifically, on running

    Code:
    ecs <- read.HTSeqCounts(sampleTable$countFile, sampleTable, dros_flattened.gff)
    I get the error:
    Code:
    Error in checkAtAssignment("character", "annotationFile", "character") :
      ‘annotationFile’ is not a slot in class “character”
    In addition: Warning message:
    'newExonCountSet' is deprecated.
    Use 'DEXSeqDataSet' instead.
    See help("Deprecated")
    I prepared the gff file using the dexseq python script, and all other steps also as in the DEXSeq vignette.

    Any suggestions/workarounds are most welcome.

    Thanks

    M

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