[ritzriya]

@thorondor - From all this, one thing for sure I've learnt is I need to know much more about the wetlab experiment also. Yes I will be assembling the reads into contigs.

@colindaven - Yes I will have to learn R for that. I was plain curious to know how one would calculate RNA-seq coverage? that's all ..

Bottom-line is (hope I've understood), is that the number of singletons you get in your assembly of reads as well as its coverage value - assesses your quality of data at the basic level of analysis.

Thanks everyone for helping!!