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Thanks Joe. It seems very confusing that they would have written so clearly in the v2 manual but this critical description is removed from the v3 manual! I wonder who made such a terrible decision to remove it. Those who pick up the v3 manual and not know of v2 would not be able to see through this. If that is the order required, it should be given in both manuals. -
In the 3' v2 protocol, 10x recommended making your mastermix, then adding water to the mastermix and pipette mixing, and then finally adding your cells to the mastermix with water.
The newest 3' v3 protocol, they do not specify an order at that step, but I have been doing it in the same order as the v2 without issue.Leave a comment:
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10x genomics scRNASeq
When one is preparing cells for the 10x genomics single cell RNA Seq platform, the manual says to dilute the cells (in media or 1x PBS) into water for a final volume of 46.6ul to achieve your targeted cell recovery. Do users mix their cell suspension in with water, then pipette into the mastermix at this step?
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Innovations in next-generation sequencing technologies and techniques are driving more precise and comprehensive exploration of complex biological systems. Current advancements include improved accessibility for long-read sequencing and significant progress in single-cell and 3D genomics. This article explores some of the most impactful developments in the field over the past year.
Long-Read Sequencing
Long-read sequencing has...12-02-2024, 01:49 PM -
The field of immunogenetics explores how genetic variations influence immune responses and susceptibility to disease. In a recent SEQanswers webinar, Oscar Rodriguez, Ph.D., Postdoctoral Researcher at the University of Louisville, and Ruben MartÃnez Barricarte, Ph.D., Assistant Professor of Medicine at Vanderbilt University, shared recent advancements in immunogenetics. This article discusses their research on genetic variation in antibody loci, antibody production processes,...11-06-2024, 07:24 PM
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