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Has anybody tried the TruSeq small RNA sample prep kit to prepare libraries for directional mRNA sequencing? We would like to multiplex some bacterial samples, and this kit should work if we fragment the RNA first.
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Pair end adapter stripping
I am using small RNA Truseq kit for pair end directional RNA seq. I am just wondering if somebody knows what adapters' sequence I can use to strip the 3' and 5' adapters. My fragments are shorter than the read length and it is important to strip off the two adapters. Thanks! -
Has anyone added the Parkhomchuck UNG step to TruSeq RNA?Comment
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Yes. I've done about 20 samples using Parkhomchuck mods with TruSeq RNA reagents and it works pretty well. I get about 93% of the reads in the correct direction.Comment
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Cancer research has been transformed through numerous molecular techniques, with RNA sequencing (RNA-seq) playing a crucial role in understanding the complexity of the disease. Maša Ivin, Ph.D., Scientific Writer at Lexogen, and Yvonne Goepel Ph.D., Product Manager at Lexogen, remarked that “The high-throughput nature of RNA-seq allows for rapid profiling and deep exploration of the transcriptome.” They emphasized its indispensable role in cancer research, aiding in biomarker...09-07-2023, 11:15 PM -
Ribonucleic acid (RNA) represents a range of diverse molecules that play a crucial role in many cellular processes. From serving as a protein template to regulating genes, the complex processes involving RNA make it a focal point of study for many scientists. This article will spotlight various methods scientists have developed to investigate different RNA subtypes and the broader transcriptome.
Whole Transcriptome RNA-seq
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