3.0.1
Yes, I'm just started it, it seems to works fine! Thnx!
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Hi,
Genomics Workbench 3.0.0 only allowed (multiple) single sequences as input for the reference(s). However, the updated version, Genomics Workbench 3.0.1, allows list(s) of references sequences as input. You can download the updated version her:
http://www.clcbio.com/index.php?id=1294
Anne-MetteLeave a comment:
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Abi Solid WT Sample Data vs. CLC Bio Genetics Workbench 3.0.0
Hi,
I'm just testing mRNA sequencing option of the CLC Genetics Workbench 3. Do you know any Abi Solid public data for it?
I have only two source: http://solidsoftwaretools.com/gf/dow...0_testData.tgz - but there isn't included qual file for proper importing it to CLC.
The other link is ftp://ftp.ncbi.nih.gov/pub/TraceDB/S...6_1_ES.tar.bz2 and ftp://ftp.ncbi.nih.gov/pub/TraceDB/S...6_2_EB.tar.bz2.
We succeed in importing, but setting annotated reference seqs failed.
We can download refseqs from ftp://ftp.ncbi.nih.gov/genomes/M_musculus/ in genbank format (*.gbk), importing to CLC is ok, but we can't choose them (one or multiple chromosomal gbk files) in the mRNA sequencing dialog. (http://www.clcbio.com/manual/genomic..._seq_step2.png) We can't see the imported refseqs at the list, where we can adding them to the analysis.
Alternatively I accept any kind NGS whole transcriptome public data (Roche 454 - sff./.fna/.qual or Illumina .txt /.fastq or Helicos (.fasta)
Thank you!
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Amplicon sequencing is a targeted approach that allows researchers to investigate specific regions of the genome. This technique is routinely used in applications such as variant identification, clinical research, and infectious disease surveillance. The amplicon sequencing process begins by designing primers that flank the regions of interest. The DNA sequences are then amplified through PCR (typically multiplex PCR) to produce amplicons complementary to the targets. RNA targets...03-21-2023, 01:49 PM -
Targeted sequencing is an effective way to sequence and analyze specific genomic regions of interest. This method enables researchers to focus their efforts on their desired targets, as opposed to other methods like whole genome sequencing that involve the sequencing of total DNA. Utilizing targeted sequencing is an attractive option for many researchers because it is often faster, more cost-effective, and only generates applicable data. While there are many approaches...03-10-2023, 05:31 AM
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