No experience with this, but I am curious how this works. I've deen doing exomes on SOLiD ald plan to do some more, So maybe this could be a good way to multiplex samples before the exome enrichment step? It would save a lot of time and money to use this instead of qpcr quant....
Can anyone share their experience with either Axy or other brands' normalization kits?
-
Since its been over a year this question was originally asked, I would like to bring this topic back and see if someone has had good/bad experience with these normalization beads? I think it would be a great option to bypass all the onerous normalization post PCR/barcoding for sequencning on either PGM or Illumina.
Thoughts?Leave a comment:
-
We still use SequalPrep normalization by Invitrogen. We haven't used the AxyPrep kit yet though.Leave a comment:
-
Amplicon normalization
I came across this this site today:
And was wondering if anyone has tried this AxyPrep Mag PCR Normalizer kit for normalizing amplicons prior to pooling for sequencing? If so, how'd it go? It seems simple and relatively straightforward, so I'm wondering why I've never heard of it before. (Or any of their other kits for that matter.)
What are people doing anyway to normalize a large number of amplicons prior to pooling and sequencing? Ampure beads/filter plate -> PicoGreen -> dilutions?
-
Qadri offers efficient online services designed for students and staff of University Targu Mures Medical Campus Hamburg. We streamline your academic and administrative processes for a hassle-free experience.
VIsit us:
https://qadri-international.com/univ...s-hamburg-umch...Yesterday, 09:33 PM -
Non-coding RNAs (ncRNAs) do not code for proteins but play important roles in numerous cellular processes including gene silencing, developmental pathways, and more. There are numerous types including microRNA (miRNA), long ncRNA (lncRNA), circular RNA (circRNA), and more. In this article, we discuss innovative ncRNA research and explore recent technological advancements that improve the study of ncRNAs.
Nobel Prize for MicroRNA Discovery
This week,...10-07-2024, 08:07 AM -
Metagenomics has improved the way researchers study microorganisms across diverse environments. Historically, studying microorganisms relied on culturing them in the lab, a method that limits the investigation of many species since most are unculturable1. Metagenomics overcomes these issues by allowing the study of microorganisms regardless of their ability to be cultured or the environments they inhabit. Over time, the field has evolved, especially with the advent...09-23-2024, 06:35 AM
Leave a comment: