No experience with this, but I am curious how this works. I've deen doing exomes on SOLiD ald plan to do some more, So maybe this could be a good way to multiplex samples before the exome enrichment step? It would save a lot of time and money to use this instead of qpcr quant....
Can anyone share their experience with either Axy or other brands' normalization kits?
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Since its been over a year this question was originally asked, I would like to bring this topic back and see if someone has had good/bad experience with these normalization beads? I think it would be a great option to bypass all the onerous normalization post PCR/barcoding for sequencning on either PGM or Illumina.
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We still use SequalPrep normalization by Invitrogen. We haven't used the AxyPrep kit yet though.Leave a comment:
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Amplicon normalization
I came across this this site today:
And was wondering if anyone has tried this AxyPrep Mag PCR Normalizer kit for normalizing amplicons prior to pooling for sequencing? If so, how'd it go? It seems simple and relatively straightforward, so I'm wondering why I've never heard of it before. (Or any of their other kits for that matter.)
What are people doing anyway to normalize a large number of amplicons prior to pooling and sequencing? Ampure beads/filter plate -> PicoGreen -> dilutions?
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Cancer research has been transformed through numerous molecular techniques, with RNA sequencing (RNA-seq) playing a crucial role in understanding the complexity of the disease. Maša Ivin, Ph.D., Scientific Writer at Lexogen, and Yvonne Goepel Ph.D., Product Manager at Lexogen, remarked that “The high-throughput nature of RNA-seq allows for rapid profiling and deep exploration of the transcriptome.” They emphasized its indispensable role in cancer research, aiding in biomarker...09-07-2023, 11:15 PM -
Ribonucleic acid (RNA) represents a range of diverse molecules that play a crucial role in many cellular processes. From serving as a protein template to regulating genes, the complex processes involving RNA make it a focal point of study for many scientists. This article will spotlight various methods scientists have developed to investigate different RNA subtypes and the broader transcriptome.
Whole Transcriptome RNA-seq
Whole transcriptome sequencing...08-31-2023, 11:07 AM
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