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Using Covaris S2 for RNA shearing

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  • #16
    I've jsut stated using the Covaris for chromatin fragmentation and so far I'm very impressed with the results. The reproducibility between samples is impressive.

    What I am wondering is what are the advantages/disavantages of using the Covaris vs. chemical methods for shearing RNA for RNA-seq library preparation?
    --------------
    Ethan

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    • #17
      Originally posted by ETHANol View Post
      I've jsut stated using the Covaris for chromatin fragmentation and so far I'm very impressed with the results. The reproducibility between samples is impressive.

      What I am wondering is what are the advantages/disavantages of using the Covaris vs. chemical methods for shearing RNA for RNA-seq library preparation?
      never got a answer for this, so what is better Covaris or the chemical/heat fragmentation? ?

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      • #18
        Physical shearing is better since it is concentration independent, isothermal, easily controllable as compared to chemical methods, and you don't have to deal with the stringent cleanup methods required after chemical/heat fragmentation.

        Thank you.

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