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  • bisulphite converted barcoded fragment library for SOLID

    Hi.
    I want to prepare barcoded fragment library for SOLID using bisulphite converted DNA. Any suggestions about modifying the multiplex adaptors in order to be suitable for this application? Can anyone provide sequences? Has anyone modified the barcodes in order to be amenable in the subsequent bisulphite conversion? Any idea/ input is highly appreciated! Thank you in advance for your help.
    Ageliki

  • #2
    Hello Ageliki,
    You might want to check out this protocol. http://www3.appliedbiosystems.com/cm...cms_089329.pdf

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    • #3
      Thanks for the suggestion.The issues are that for the barcoding some modifications are required. So the multiplex P1 adaptor that should be the methylated one should be much shorter than the P1 adaptor suggested in the link in order to compensate for the longer multiplex P2 adaptor and also most probably the barcodes will be affected by the bs treatment.
      Ageliki

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