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We want to use the ScriptSeq v2 RNA-seq Library prep kit (Epicentre) to make libraries for some rRNA-depleted bacterial RNA. Looking through the protocol I don't see any option for a size-selection. We'd like to size-select because we have a lot of small RNA product that we don't want to include in the prep. Has anyone incorporated a size-selection into the protocol? I would think you could add it after the completed cDNA synthesis and tagging (after 3.C in current protocol). Would you still do the cDNA purification (3.D in current protocol) if there is a size-selection after cDNA synthesis? We have access to a pippin prep if that will be useful.
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Cancer research has been transformed through numerous molecular techniques, with RNA sequencing (RNA-seq) playing a crucial role in understanding the complexity of the disease. Maša Ivin, Ph.D., Scientific Writer at Lexogen, and Yvonne Goepel Ph.D., Product Manager at Lexogen, remarked that “The high-throughput nature of RNA-seq allows for rapid profiling and deep exploration of the transcriptome.” They emphasized its indispensable role in cancer research, aiding in biomarker...09-07-2023, 11:15 PM -
Ribonucleic acid (RNA) represents a range of diverse molecules that play a crucial role in many cellular processes. From serving as a protein template to regulating genes, the complex processes involving RNA make it a focal point of study for many scientists. This article will spotlight various methods scientists have developed to investigate different RNA subtypes and the broader transcriptome.
Whole Transcriptome RNA-seq
Whole transcriptome sequencing...08-31-2023, 11:07 AM