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  • BioDynami
    replied
    There is a kit for ssDNA: NGS Single Stranded DNA Library Prep Kit from BioDynami:
    https://www.biodynami.com/ngs_single...brary_prep_kit

    Leave a comment:


  • Genohub
    replied
    ssDNA ligation

    Check out this article, it should help: Single-stranded DNA library preparation for the sequencing of ancient or damaged DNA.

    If you don't follow the approach in the paper, you can use T4 Polynucleotide Kinase to add a 5'- Phosphate and remove 3ยด-phosphoryl groups.


    - Genohub

    Leave a comment:


  • dtm2451
    started a topic Library prep with sheared ssDNA

    Library prep with sheared ssDNA

    Does anyone know the integrity/makeup of the 5' and 3' ends of Covaris-sheared ssDNA? I am working on a library prep method for single stranded cDNA and am hoping to ligate adapters post shearing.

    My assumption is that the cleavage could not leave intact 5' phosphate and 3' OH. Therefore, I am also wondering about methods to repair these ends that would not require generation of a second strand.

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