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  • Ribosomal cDNA depletion

    If I understand correctly, a lot of the kits for ribosomal RNA depletion kits use antisense oligos to bind and deplete ribosomal RNAs.

    Would it be possible to alternatively deplete ribosomal cDNAs instead?

  • #2
    You can deplete rRNA at the cDNA level if you use a technique like DSN (double-stranded nuclease) or HAC (hydroxyapatite chromatography). We published a paper a while back about HAC, showing high levels of rRNA depletion using both techniques.

    Second-generation sequencing (SGS) has become the preferred method for RNA transcriptome profiling of organisms and single cells. However, SGS analysis of transcriptome diversity (including protein-coding transcripts and regulatory non-coding RNAs) is inefficient unless the sample of interest is fir …

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    • #3
      Hi!
      You can try the PureRec Duplex-Specific Nuclease (DSN) from Zymo Research (E1020S, E1020-200, E1020-1000). It can reduce abundant rRNA and globin transcripts for NGS libraries and is selective to digest only dsDNA while leaving the ssDNA or RNA alone. Check it out and I hope it helps!

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