Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Lindsey
    replied
    I know it's not really an answer to your question, but I think that this figure is very helpful to understand bead purification.

    Leave a comment:


  • SethDennison
    replied
    From what I understand about the SPB beads, the behavior of the DNA is dependent on the concentration of PEG and salt in the reaction, the volumetric ratio of beads to DNA is critical. Part of the reason for this effect is that DNA fragment size affects the total charge per molecule with larger DNAs having larger charges; this promotes their electrostatic interaction with the beads and displaces smaller DNA fragments. When you decrease the ratio there only larger DNA fragments will be allowed to bond with the beads while if you you increase the ratio smaller DNA fragments have the chance to bond.

    Leave a comment:


  • the principle of double-sided size selection of Illumina DNA prep bead clean-up?

    I don't fully understand the principle of double-sided size selection of Illumina DNA prep bead clean-up. Can someone explain to me?
    I know the first clean-up is using diluted SPB beads to remove right-side large fragments and the second clean-up is using undiluted SPB beads to remove left-side small fragments.
    I also know in both clean-up steps, the larger fragments are bound to beads. In the first clean-up, we keep the supernatant which contains the smaller fragments and in the second clean-up we keep the beads which contains the larger fragments.
    What I do not know is why the fragments larger than a certain size are bound to the diluted beads and the fragment larder than another size are bound to the undiluted beads,

Latest Articles

Collapse

  • seqadmin
    Strategies for Sequencing Challenging Samples
    by seqadmin


    Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...
    03-22-2024, 06:39 AM
  • seqadmin
    Techniques and Challenges in Conservation Genomics
    by seqadmin



    The field of conservation genomics centers on applying genomics technologies in support of conservation efforts and the preservation of biodiversity. This article features interviews with two researchers who showcase their innovative work and highlight the current state and future of conservation genomics.

    Avian Conservation
    Matthew DeSaix, a recent doctoral graduate from Kristen Ruegg’s lab at The University of Colorado, shared that most of his research...
    03-08-2024, 10:41 AM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by seqadmin, Yesterday, 06:37 PM
0 responses
10 views
0 likes
Last Post seqadmin  
Started by seqadmin, Yesterday, 06:07 PM
0 responses
9 views
0 likes
Last Post seqadmin  
Started by seqadmin, 03-22-2024, 10:03 AM
0 responses
50 views
0 likes
Last Post seqadmin  
Started by seqadmin, 03-21-2024, 07:32 AM
0 responses
67 views
0 likes
Last Post seqadmin  
Working...
X