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Hello,
We plan to perform Expressed Exome Capture Sequencing (EecSeq) (Puritz and Lotterhos 2018) experiment that includes duplex-specific nuclease (DSN) normalization of cDNA libraries. The DSN kit is produced by Evrogen company (cat.# EA001) that is based in Russia, and there are currently no options to purchase the reagent due to the war embargo. After checking all available options I still don't have an idea where I could get it. Maybe there is someone out there who could share the kit now so I can rebuy it when it's possible?
Or maybe someone has experience how the normalization step can be replaced?
We plan to perform Expressed Exome Capture Sequencing (EecSeq) (Puritz and Lotterhos 2018) experiment that includes duplex-specific nuclease (DSN) normalization of cDNA libraries. The DSN kit is produced by Evrogen company (cat.# EA001) that is based in Russia, and there are currently no options to purchase the reagent due to the war embargo. After checking all available options I still don't have an idea where I could get it. Maybe there is someone out there who could share the kit now so I can rebuy it when it's possible?
Or maybe someone has experience how the normalization step can be replaced?