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  • RNA Extraction from Frozen EDTA Blood

    Is there anyone who can isolate RNA from frozen EDTA blood sample or develop a method? I am open to all kits and methods on this subject.

  • #2
    It has been several years since I've had to do this, but I remember using the QIAamp DNA Blood Kits and it worked fine. I also had a colleague use another kit from Thermo that worked well but I don't remember the name.

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    • #3
      What you say is correct. QIAamp DNA Blood works on frozen blood. But I need it for RNA. I always came across a warning in QIAgen kits such as "Frozen blood samples are not recommended for RNA." The Nucleospin RNA kit will arrive within 1 week. I will try that kit for my optimization work.
      If this kit does not work, I will try changing a few methods or combining the steps of some protocols. By adding things like that water bath, aluminum, heat block etc.

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      • #4
        Hi Mehmet, did you get any good results so far? I have been trying to work with frozen EDTA samples in the last weeks and am now planning to do single nucleus sequencing, hoping are chances to get relatively high RNA yield. I have also tried aluminum thawing and found this publication for improved quality: https://www.nature.com/articles/s415...Rec=false#Sec9.

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