Hi Everyone,
I have been extracting RNA from Drosophila wing imaginal discs for bulk sequencing. When I have a core run my RNA on a Qubit and Tapestation, they say the samples "are technically below detection on the tape station". They have not ran RNA from insects, would this interpretation of theirs be due to the 28s being split in drosophila?
For reference, the Qubit shows this sample has 7.6ng/ul in a total of 40ul
I am mainly looking for input on:
I have been extracting RNA from Drosophila wing imaginal discs for bulk sequencing. When I have a core run my RNA on a Qubit and Tapestation, they say the samples "are technically below detection on the tape station". They have not ran RNA from insects, would this interpretation of theirs be due to the 28s being split in drosophila?
For reference, the Qubit shows this sample has 7.6ng/ul in a total of 40ul
I am mainly looking for input on:
- if the quality of my RNA is sufficient to send off for bulk sequencing
- simple tips to interpret this
- if the large peak around 25 nt is all of my sample degraded?
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