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  • concentration mRNA required

    Hello everybody!

    i'm struggling with the RNA extraction from bacteria recovered from mice after intraperitoneal infection.... unfortunately i'm not able to get a good amount of total RNA ( the best sample is 256 ng/microliter) and i have around 15 microliter of the sampke) and i'm afraid that the concentration will decrease a lot after the rRNA removal treatment with RiboZero rRNA removal kit.
    Does anyone know the concentration of mRNA required for the mRNA sequencing on the Illumina platform?

    thank you

  • #2
    The Epicentre ScriptSeq kit uses starting amounts 0f 500pg - 50ng of rRNA depleted RNA. I think you are in the ballpark.

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    • #3
      ok, thank you very much! fingers cross!

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