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  • Low throughput DNA quantitation?

    I need 200ng and 50ng/ul DNA for my downstream application. I only need to process eight samples a day. What DNA quantitation method should I use?

    Is Qubit fluorometer good enough for me???

  • #2
    Both Illumina and ABI recommend Qubit over NanoDrop for quantifying DNA.

    Comment


    • #3
      I heartily recommend Qubit as well.
      Has anyone seen this recent piece concerning plastic leaching and DNA quantitation:
      http://www.nature.com/news/2010/1004....2010.200.html

      The nice thing about the Qubit is it doesn't see anything but dsDNA, unlike the nanodrop.

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      • #4
        The Qubit is good, the readings are slightly higher than using a bioanalyser but the results are repeatable and much closer to reality than a nanodrop.

        The nanodrop is only useful for reading circularised DNA for SOlid long mate pair but it's still not great for this.

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        • #5
          The libraries I made using NanoDrop values were all very good. We bought the Qubit since it's recommended but haven't made libraries using it's values yet.

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          • #6
            Also, at least for protein quantification you can't use common protein buffers to dissolve your proteins in on the Qubit. You have to use their proprietary buffer and they won't even tell you what's in it.

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            • #7
              Nice. Thank you all for your replies. I think I will go with Qubit then.

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