Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • kerplunk412
    replied
    Scaling up a reaction volume will not result in more library, unless you scale up the input accordingly. Pretty much all NGS kits should already get you much more library than you need for sequencing if you use the highest recommended input, so there wouldn't be much point in scaling up a reaction, in my opinion.

    Scaling down a reaction could be performed, but it probably will not allow you to go below the recommended input. The biggest advantage of scaling down is saving money. Scaling down may require some slight optimization, but if you have a lot of samples to prep it may be worth the optimization if you can make a kit last twice as long.

    Leave a comment:


  • Nighthawkrao77
    started a topic Scale Up or Down Reaction Volumes?

    Scale Up or Down Reaction Volumes?

    Has anyone used TruSeq RNA or NEBNext RNA kits and tried to scale the volumes down or up? Is this even possible? Just curious to see if I can add more to generate more libraries, or less to proportionally add less RNA input.

Latest Articles

Collapse

  • seqadmin
    Non-Coding RNA Research and Technologies
    by seqadmin


    Non-coding RNAs (ncRNAs) do not code for proteins but play important roles in numerous cellular processes including gene silencing, developmental pathways, and more. There are numerous types including microRNA (miRNA), long ncRNA (lncRNA), circular RNA (circRNA), and more. In this article, we discuss innovative ncRNA research and explore recent technological advancements that improve the study of ncRNAs.

    [Article Coming Soon!]...
    Today, 08:07 AM
  • seqadmin
    Recent Developments in Metagenomics
    by seqadmin





    Metagenomics has improved the way researchers study microorganisms across diverse environments. Historically, studying microorganisms relied on culturing them in the lab, a method that limits the investigation of many species since most are unculturable1. Metagenomics overcomes these issues by allowing the study of microorganisms regardless of their ability to be cultured or the environments they inhabit. Over time, the field has evolved, especially with the advent...
    09-23-2024, 06:35 AM
  • seqadmin
    Understanding Genetic Influence on Infectious Disease
    by seqadmin




    During the COVID-19 pandemic, scientists observed that while some individuals experienced severe illness when infected with SARS-CoV-2, others were barely affected. These disparities left researchers and clinicians wondering what causes the wide variations in response to viral infections and what role genetics plays.

    Jean-Laurent Casanova, M.D., Ph.D., Professor at Rockefeller University, is a leading expert in this crossover between genetics and infectious...
    09-09-2024, 10:59 AM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by seqadmin, 10-02-2024, 04:51 AM
0 responses
14 views
0 likes
Last Post seqadmin  
Started by seqadmin, 10-01-2024, 07:10 AM
0 responses
24 views
0 likes
Last Post seqadmin  
Started by seqadmin, 09-30-2024, 08:33 AM
1 response
31 views
0 likes
Last Post EmiTom
by EmiTom
 
Started by seqadmin, 09-26-2024, 12:57 PM
0 responses
19 views
0 likes
Last Post seqadmin  
Working...
X