Tn5

[genefuse]

Does anyone know of another source of Tn5 transposase other than
FC 121 1030 form Illumina.
Cheers

[Olaf Blue]

Epicentre's EZ-Tn5 Transposase; TNP92110, which is a special order item.

[genefuse]

Thank you for the suggestion. Can Epicentre's EZ-Tn5 TNP92110 completely substitute for the Illumina product in terms of primer sequence, buffers, reaction conditions, etc.
Appreciated,
Genefuse

[Olaf Blue]

Happy to help - this is just the bare enzyme and users have to load any oligos themselves. Here's the product page link:

http://www.epibio.com/enzymes/in-viv...posase?details

Reaction buffer and stop solution is provided; we don't do custom oligo loading.

Disclaimer: I am part of ILMN in Madison, WI

[genefuse]

Great!
Much appreciated
Genefuse

[irene_chen]

Hi, gene fuse
Have you tried the EZ-Tn5 from epicenter? Could it be used for DNA library preparation?

[genefuse]

Tn5

Hi,
I have not had a chance to try EN-Tn5.
Olaf Blue might have more info on this
Genefuse

[Olaf Blue]

EZ-Tn5 from Epicentre has been transferred for sales and support to Lucigen:

http://www.lucigen.com/EZ-Tn5-Transposase/

Olaf

[irene_chen]

I am finding the replacement of Nextera from Illumina but I am not sure if it could be used for tagmentation. It seems to be mostly used for the gene mutation.