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  • Tn5

    Does anyone know of another source of Tn5 transposase other than
    FC 121 1030 form Illumina.
    Cheers

  • #2
    Epicentre's EZ-Tn5 Transposase; TNP92110, which is a special order item.

    Comment


    • #3
      Thank you for the suggestion. Can Epicentre's EZ-Tn5 TNP92110 completely substitute for the Illumina product in terms of primer sequence, buffers, reaction conditions, etc.
      Appreciated,
      Genefuse

      Comment


      • #4
        Happy to help - this is just the bare enzyme and users have to load any oligos themselves. Here's the product page link:

        http://www.epibio.com/enzymes/in-viv...posase?details

        Reaction buffer and stop solution is provided; we don't do custom oligo loading.

        Disclaimer: I am part of ILMN in Madison, WI

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        • #5
          Great!
          Much appreciated
          Genefuse

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          • #6
            Hi, gene fuse
            Have you tried the EZ-Tn5 from epicenter? Could it be used for DNA library preparation?

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            • #7
              Tn5

              Hi,
              I have not had a chance to try EN-Tn5.
              Olaf Blue might have more info on this
              Genefuse

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              • #8
                EZ-Tn5 from Epicentre has been transferred for sales and support to Lucigen:

                http://www.lucigen.com/EZ-Tn5-Transposase/

                Olaf

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                • #9
                  I am finding the replacement of Nextera from Illumina but I am not sure if it could be used for tagmentation. It seems to be mostly used for the gene mutation.

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