Hi,
we're planning to investigate the transcriptome of a conifer species. It is intended to construct 3' directed cDNA libraries, preferentially by random fragmentation that will be sequenced on the Illumina Genome Analyzer. I found some protocols, especially this http://www.plosone.org/article/info%...l.pone.0008768 one appeared very suitable.
The question is, if somebody of you has made any experience with random 3' directed cdna libraries on the Illumina Genome Analyzer and maybe know some good working protocols.
Thanks in Advance
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