Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • nucacidhunter
    replied
    If there is no primer dimer at the end point of qPCR reactions using KAPA standards and Illumina primers then it would be safe to use them.

    Leave a comment:


  • hoytpr
    replied
    Fair enough. KAPA doesn't seem to know for certain, so it'll remain a mystery.

    Leave a comment:


  • hoytpr
    started a topic Can you use Illumina qPCR primers with KAPA

    Can you use Illumina qPCR primers with KAPA

    Sorry if this has been asked before, I searched and searched, couldn't find the exact answer.

    We have HPLC-purified Illumina qPCR primers, but want to order the KAPA qPCR standards kit (because we already have the KAPA qPCR master mix). One primer is the same for both Illumina and KAPA protocols, but the other primer is 3bp shorter in the KAPA kit. Has anyone used the standard Illumina qPCR primers in place of the KAPA qPCR primers (for the KAPA standards)?

    Thanks.

Latest Articles

Collapse

  • seqadmin
    Strategies for Sequencing Challenging Samples
    by seqadmin


    Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...
    03-22-2024, 06:39 AM
  • seqadmin
    Techniques and Challenges in Conservation Genomics
    by seqadmin



    The field of conservation genomics centers on applying genomics technologies in support of conservation efforts and the preservation of biodiversity. This article features interviews with two researchers who showcase their innovative work and highlight the current state and future of conservation genomics.

    Avian Conservation
    Matthew DeSaix, a recent doctoral graduate from Kristen Ruegg’s lab at The University of Colorado, shared that most of his research...
    03-08-2024, 10:41 AM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by seqadmin, Yesterday, 06:37 PM
0 responses
10 views
0 likes
Last Post seqadmin  
Started by seqadmin, Yesterday, 06:07 PM
0 responses
9 views
0 likes
Last Post seqadmin  
Started by seqadmin, 03-22-2024, 10:03 AM
0 responses
49 views
0 likes
Last Post seqadmin  
Started by seqadmin, 03-21-2024, 07:32 AM
0 responses
67 views
0 likes
Last Post seqadmin  
Working...
X