Is anyone using SPRI beads other than Beckman Coulter's Agencourt AMPure XP? Is anyone using MagBio's HighPrep PCR beads?
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We just follow the DIY SPRI bead protocol here https://openwetware.org/wiki/SPRI_bead_mix. We tested our home-brewed mixture with Ampure beads and didn't find much difference.
It is extremely cheap to make compared to the Ampure beads. You could make a liter of it for 400 bucks.
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PCR Clean DX
These are from Aline Biosciences.
In my last lab our robotic company tried to tell me our preps weren't working because we used Aline. They sent us Ampure beads and we did a side-by-side comparison. Aline performed better and at about 1/3 the cost.
To sum up: YES! Use them! They are great and a good company to work with as well.
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SPRI Beads for DNA, RNA, microRNA & Oligo Purification
SPRI Beads (DNA & RNA Purification)
Optimized to purify DNA fragments of 100 bp and larger, and RNA fragments of 200 bases and larger.
Features:
Purification of DNA and RNA• DNA fragments > 100 bp Applications:
• RNA fragments > 200 nt• NGS Library preparation
• PCR fragment purification
• DNA and RNA fragment purification
• Other applications requiring purified DNA and RNA
SPRI Beads (microRNA & Oligo Purification)
Overcomes the hurdle of the short DNA/RNA recovery problem. DNA/RNA fragments shorter than 100 base pairs can be effectively recovered.
Features:
Purification of short DNA and RNA• microRNA
• dsDNA fragments >20 bp
• ssDNA fragments >20 nt
• RNA fragments >20 nt
• DNA/RNA hybrid fragments >20 nt
• Oligo and chimeric oligo >20 nt
Learn More
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SPRI Beads (DNA & RNA Purification)
Optimized to purify DNA fragments of 100 bp and larger, and RNA fragments of 200 bases and larger.
Features:
Purification of DNA and RNA• DNA fragments > 100 bp Applications:
• RNA fragments > 200 nt• NGS Library preparation
• PCR fragment purification
• DNA and RNA fragment purification
• Other applications requiring purified DNA and RNA
SPRI Beads (microRNA & Oligo Purification)
Overcomes the hurdle of the short DNA/RNA recovery problem. DNA/RNA fragments shorter than 100 base pairs can be effectively recovered.
Features:
Purification of short DNA and RNA• microRNA
• dsDNA fragments >20 bp
• ssDNA fragments >20 nt
• RNA fragments >20 nt
• DNA/RNA hybrid fragments >20 nt
• Oligo and chimeric oligo >20 nt
Learn More
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Hello everyone,
MagBio Genomics offers HighPrep PCR clean-up kit, a direct replacement to AMPure XP beads that is over 50% cheaper. No change to protocol is needed. Please reach out to me at [email protected] to inquire more.
Thank you.
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DINOMAG cleanup and size selection SPRI magenetic beads (cat# DN9004-75ML) works great as a direct replacement to AMPure XP at a much lower price! https://labscoop.com/us/en/product/r...magnetic-beads (probably ~50-80% less than other commercially available magnetic bead-based reagents listed)Last edited by carlw; 01-23-2024, 06:18 PM.
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