Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • RNAseq library for polysome profiling (monosome and polysome-associated RNA)

    Hi there,

    I am interested in performing polysome profiling with downstream application for RNAseq of monosome and polysome-associated RNAs.

    Has anyone had much experience with choice of library preparations for monosome and polysome-associated RNAs?

    ribosomal RNA surely is to be more abundant in these samples compared to total RNA extractions? This would lead me towards preferring ribosome-depletion (e.g. riboZero) as opposed to polyA. However PolyA is better for intact fragments and we also intend to sequence total RNA where polyA would be preferred. If we want to compare sequencing between total RNA and mono/poly RNAs it would make sense to use the same library prep?

    If you have any experience in choice of library prep (poly A vs ribo-depletion) for polysome-profiling RNAseq experiments please let me know which method you preferred and why

    Many thanks,

Latest Articles

Collapse

  • seqadmin
    Exploring the Dynamics of the Tumor Microenvironment
    by seqadmin




    The complexity of cancer is clearly demonstrated in the diverse ecosystem of the tumor microenvironment (TME). The TME is made up of numerous cell types and its development begins with the changes that happen during oncogenesis. “Genomic mutations, copy number changes, epigenetic alterations, and alternative gene expression occur to varying degrees within the affected tumor cells,” explained Andrea O’Hara, Ph.D., Strategic Technical Specialist at Azenta. “As...
    07-08-2024, 03:19 PM
  • seqadmin
    Exploring Human Diversity Through Large-Scale Omics
    by seqadmin


    In 2003, researchers from the Human Genome Project (HGP) announced the most comprehensive genome to date1. Although the genome wasn’t fully completed until nearly 20 years later2, numerous large-scale projects, such as the International HapMap Project and 1000 Genomes Project, continued the HGP's work, capturing extensive variation and genomic diversity within humans. Recently, newer initiatives have significantly increased in scale and expanded beyond genomics, offering a more detailed...
    06-25-2024, 06:43 AM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by seqadmin, Yesterday, 07:20 AM
0 responses
19 views
0 likes
Last Post seqadmin  
Started by seqadmin, 07-16-2024, 05:49 AM
0 responses
36 views
0 likes
Last Post seqadmin  
Started by seqadmin, 07-15-2024, 06:53 AM
0 responses
39 views
0 likes
Last Post seqadmin  
Started by seqadmin, 07-10-2024, 07:30 AM
0 responses
41 views
0 likes
Last Post seqadmin  
Working...
X