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Fragment Analyser HS NGS Frag 1-6000 profile

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  • Fragment Analyser HS NGS Frag 1-6000 profile

    I'm looking for some help analysing the profile of a 10x genomics 3' scRNA library prep, run on a 1-6000bp HS fragment analyser (attached pic). The second of my libraries doesn't have a 'smooth' profile and I was hoping for some insight as to why this might be! Will it affect sequencing?

    For reference, this data was obtained from differentiating hiPSCs at different time-points.

    Thanks!



    https://ibb.co/jM0yqQ7
    Last edited by Sbiljh1; 12-07-2021, 07:25 AM.

  • #2
    Hello,

    I am having the same profile of the second fragment analyzer peek, where it is split into two.

    Did you manage to find any answer or good interpretation?

    thank you!

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