Hi all
I recently made ATAC-seq libraries on two new cancer cell lines and I don't see nucleosomal pattern as I used to see in my previous library. They look either over- or under- tagmented. Any suggestion about what goes wrong and how to optimize the tagmentation is appreciated !
I recently made ATAC-seq libraries on two new cancer cell lines and I don't see nucleosomal pattern as I used to see in my previous library. They look either over- or under- tagmented. Any suggestion about what goes wrong and how to optimize the tagmentation is appreciated !