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Sample Prep / Library Generation
Techniques and protocol discussions on sample preparation, library generation, methods and ideas
Topics: 2,181
Posts: 9,846
Last Post:
qPCR for library quantification
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2,181 | 9,846 |
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Single Cell Sequencing
Techniques, discussions, and applications of single cell sequencing.
Topics: 3
Posts: 7
Last Post:
PHLAT genotyping with scRNA-seq (10x)
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3 | 7 |
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Genomic Resequencing
Variant discovery in previously sequenced genomes/regions
Topics: 277
Posts: 990
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277 | 990 |
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De novo discovery
Wandering without a reference? Post here
Topics: 341
Posts: 1,700
Last Post:
infering isoforms in merged transcriptome
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341 | 1,700 |
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Metagenomics
Ever wonder what's growing in that hot spring or glacier?
Topics: 267
Posts: 840
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267 | 840 |
by GenomicSeq
05-11-2023, 03:06 PM
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Epigenetics
Any non-primary sequence heritable modification of genetic material. ChIP-SEQ, DNA methylation (Bisulfite-SEQ), chromatin modifications (methylation, acetylation, etc), non coding RNA.
Topics: 356
Posts: 1,577
Last Post:
DMRcaller Minimum Reads per Cytosine
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356 | 1,577 |
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RNA Sequencing
Application of sequencing to RNA analysis (RNA-Seq, whole transcriptome, SAGE, expression analysis, novel organism mining, splice variants)
Topics: 2,850
Posts: 11,391
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2,850 | 11,391 |
by GenomicSeq
05-25-2023, 06:49 AM
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Clinical Sequencing
Discuss issues unique to clinical sequencing.
Topics: 39
Posts: 104
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39 | 104 |
by petch
11-13-2022, 09:42 AM
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Differential Expression and Data Visualization: Recommended Tools for Next-Level Sequencing Analysis
After covering QC and alignment tools in the first segment and variant analysis and genome assembly in the second segment, we’re wrapping up with a discussion about tools for differential gene expression analysis and data visualization. In this article, we include recommendations from the following experts: Dr. Mark Ziemann, Senior Lecturer in Biotechnology and Bioinformatics, Deakin University; Dr. Medhat Mahmoud Postdoctoral Research Fellow at Baylor College of Medicine;...05-23-2023, 12:26 PM -
Continuing from our previous article, we share variant analysis and genome assembly tools recommended by our experts Dr. Medhat Mahmoud, Postdoctoral Research Fellow at Baylor College of Medicine, and Dr. Ming "Tommy" Tang, Director of Computational Biology at Immunitas and author of From Cell Line to Command Line.
Variant detection and analysis tools
Mahmoud classifies variant detection work into two main groups: short variants (<50...05-19-2023, 10:03 AM -
With new tools and computational resources being released regularly, it can be hard to determine which are best suited for the analysis process and which older tools continue to be maintained. In an effort to assist the sequencing community, we interviewed three highly skilled bioinformaticians about their recommended tools for several important analysis applications.
Quality control and preprocessing tools
“Garbage in, garbage out” is a popular...05-16-2023, 10:11 AM