Hi all,
I have some pair-end RNA-Seq datasets, 50bp*2 with 200bp inserts. Now I have mapped them with Tophat, and my question is, how should I manipulate the mapped reads in bam file to distinguish signal on the opposite strand from the paired reads that mapped on the opposite strand? For example, a gene on the + strand is detected with expression, then it will have paired reads mapped on the - strand about 200bp downstream from the reads that mapped on the + strand, then how do I distinguish these reads from a real gene on the - strand and is also expressed? Is there any specialized tool to do this? Or any genome browser can visualize PE reads?
-
This year’s Advances in Genome Biology and Technology (AGBT) General Meeting commemorated the 25th anniversary of the event at its original venue on Marco Island, Florida. While this year’s event didn’t include high-profile musical performances, the industry announcements and cutting-edge research still drew the attention of leading scientists.
The Headliner
The biggest announcement was Roche stepping back into the sequencing platform market. In the years since...03-03-2025, 01:39 PM -
The human gut contains trillions of microorganisms that impact digestion, immune functions, and overall health1. Despite major breakthroughs, we’re only beginning to understand the full extent of the microbiome’s influence on health and disease. Advances in next-generation sequencing and spatial biology have opened new windows into this complex environment, yet many questions remain. This article highlights two recent studies exploring how diet influences microbial...02-24-2025, 06:31 AM