Hi everyone
I used bwa to align reads of multiple lane against genome,
Here I have a question.
For example,
If I have s_1_1_sequence.sai and s_1_2_sequence.sai and.. s_1_6_sequence.sai
Could I merge these sai file to s_1_total_sequence.sai ,and running bwa samse to convert to sam file ?
If I can't do this ,I must covert all sai sam independently .
And How could I use samtools merge six sam file to one total.sam
I want to know the usage of command.
Thank!!!
I used bwa to align reads of multiple lane against genome,
Here I have a question.
For example,
If I have s_1_1_sequence.sai and s_1_2_sequence.sai and.. s_1_6_sequence.sai
Could I merge these sai file to s_1_total_sequence.sai ,and running bwa samse to convert to sam file ?
If I can't do this ,I must covert all sai sam independently .
And How could I use samtools merge six sam file to one total.sam
I want to know the usage of command.
Thank!!!
Comment