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It's due to a difference in the library prep. Directional RNA-seq keeps the strand information by only making the cDNA in one direction, non-directional will make cDNA in both directions, so you have no way of knowing if your sequence read is in the same direction as the original transcription. -
directional vs. non-directional sequencing
Hi,
Can someone please explain the difference between the directional and the non-directional sequencing methods?
I was looking for articles or sites which can explain it, especially the results one get from such run.
We have our results. This suppose to be a non-directional RNA-seq. The question was now, if we can extract the strand information from these data. I mean, is there a way to know if a strand belongs to the + or - strand?
In the bam file we have these results, but as far as I understand it, this information says only if the read was mapped to the forward or backward strand.
Am I wrong with this assumption?
I would appreciate ant information which will help me/us better understand the difference between the two technologies.
Thanks
A.
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The introduction of single-cell sequencing has advanced the ability to study cell-to-cell heterogeneity. Its use has improved our understanding of somatic mutations1, cell lineages2, cellular diversity and regulation3, and development in multicellular organisms4. Single-cell sequencing encompasses hundreds of techniques with different approaches to studying the genomes, transcriptomes, epigenomes, and other omics of individual cells. The analysis of single-cell sequencing data i
...01-24-2023, 01:19 PM -
Single-cell sequencing is a technique used to investigate the genome, transcriptome, epigenome, and other omics of individual cells using high-throughput sequencing. This technology has provided many scientific breakthroughs and continues to be applied across many fields, including microbiology, oncology, immunology, neurobiology, precision medicine, and stem cell research.
The advancement of single-cell sequencing began in 2009 when Tang et al. investigated the single-cell transcriptomes...01-09-2023, 03:10 PM
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