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directional vs. non-directional sequencing

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  • directional vs. non-directional sequencing

    Hi,

    Can someone please explain the difference between the directional and the non-directional sequencing methods?

    I was looking for articles or sites which can explain it, especially the results one get from such run.

    We have our results. This suppose to be a non-directional RNA-seq. The question was now, if we can extract the strand information from these data. I mean, is there a way to know if a strand belongs to the + or - strand?
    In the bam file we have these results, but as far as I understand it, this information says only if the read was mapped to the forward or backward strand.

    Am I wrong with this assumption?

    I would appreciate ant information which will help me/us better understand the difference between the two technologies.

    Thanks
    A.

  • #2
    It's due to a difference in the library prep. Directional RNA-seq keeps the strand information by only making the cDNA in one direction, non-directional will make cDNA in both directions, so you have no way of knowing if your sequence read is in the same direction as the original transcription.

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