Hi there,
I would like to assemble a bacterial transcriptome using cufflinks (with reference genome). Does anyone have any suggestions on the parameters "--overhang-tolerance"--number of terminal exon bp to tolerate in introns, and "--min-intron-length"--minimum intron size allowed in genome?
How to set these parameters to adjust to the bacteria case? Thanks in advance!
I would like to assemble a bacterial transcriptome using cufflinks (with reference genome). Does anyone have any suggestions on the parameters "--overhang-tolerance"--number of terminal exon bp to tolerate in introns, and "--min-intron-length"--minimum intron size allowed in genome?
How to set these parameters to adjust to the bacteria case? Thanks in advance!