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  • Command line blast with remote option

    Hello,
    I am trying to run blastn from my command line with the -remote option, and I get a proxy error as

    Error: (311.26) SOCK#1000[6]@130.14.29.110:80: [SOCK::Connect] Failed pending connect(): Closed {error=111,Connection refused}
    Error: (303.7) [URL_Connect] Failed to connect to www.ncbi.nlm.nih.gov:80: Closed
    Error: (311.26) SOCK#2000[6]@130.14.29.110:80: [SOCK::Connect] Failed pending connect(): Closed {error=111,Connection refused}
    ...

    Any idea how to fix this please?
    Thanks

  • #2
    It is a network problem, so it could go away if you retry at a less busy time. Can you use BLAST from your browser? Is there a proxy in the way? If so, you might need to talk to your network administrator.

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    • #3
      It is a proxy error, I think. I was wondering if there is a proxy option in blastn itself. I can run it from the browser. But I can also run it directly from applications like clcBio. So the configuration can be done - just not sure how to do it for blastn.
      Am getting my network people to look into it also..
      Thanks so much!

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