Tweet
DNA sequencers 150 bps Output File
I am doing some experiment using BowTie 2. Now, I want, to experiment with it on 150 bps reads. So, I want DNA sequencers 150 bps output file. For example, output of Genome Analyzer IIx. Can anybody please help me, where can I find it ?
-
-
I think your best bet would be to find a MiSeq data set (rather than a GAIIx). MiSeq will easily do 2 x 150 bp. There seem to be MiSeq runs several available in the SRA (http://sra.dnanexus.com/?result_type...&show=&q=miseq or http://www.ncbi.nlm.nih.gov/sra?term=miseq). Not simple to determine if they are 150 bp so you may need to look through some. -
Thanks a lot. I go through those links. In their download section they have not specify number of bases (means 100 or 150 etc.). You mean to download and check number of bases ?Comment
-
I *think* you can actually see the length of the reads by clicking on the names of the SRA records.
See the example below. This seems to be a 150 bp paired-end MiSeq run. But them many of the others seem to be as well.
http://www.ncbi.nlm.nih.gov/sra/SRX149549
Originally posted by Arupsss View PostComment
-
If you need human samples (rather than bacteria) : http://www.ncbi.nlm.nih.gov/sra?term=miseq%20humanComment
-
There are also a couple of default 150PE runs if you're registered on Illumina BaseSpace.
Just a PhiX and BacillusCereus run, so I'm not sure how useful that'll be.
FASTQ is downloadable.Comment
-
Amplicon sequencing is a targeted approach that allows researchers to investigate specific regions of the genome. This technique is routinely used in applications such as variant identification, clinical research, and infectious disease surveillance. The amplicon sequencing process begins by designing primers that flank the regions of interest. The DNA sequences are then amplified through PCR (typically multiplex PCR) to produce amplicons complementary to the targets. RNA targets...03-21-2023, 01:49 PM -
Targeted sequencing is an effective way to sequence and analyze specific genomic regions of interest. This method enables researchers to focus their efforts on their desired targets, as opposed to other methods like whole genome sequencing that involve the sequencing of total DNA. Utilizing targeted sequencing is an attractive option for many researchers because it is often faster, more cost-effective, and only generates applicable data. While there are many approaches...03-10-2023, 05:31 AM
Comment