Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • maubp
    replied
    You need a BLAST database, usually created from a big FASTA file of all the target sequences with the makeblastdb command (or formatdb in the old legacy BLAST tool suite). A BLAST database is several files with a common name. The filenames will be name.p* for a protein database (including name.psq), and name.n* for a nucleotide database, but at the command line you just use the name without any extension. So probably:

    blastp -db <in-house database> -evalue 0.001 -i <file name> -o <file name>

    Leave a comment:


  • Blasting your blastx results against your own database?

    Hi! I have .xml, .html, .art file results form a blastx. I need to blastp one of these files against an in-house database.
    Do you know which files (.xml, .html, or .art) I should blast?

    And what would the unix command be?

    blastp -db <in-house database.psq> -evalue 0.001 -i <file name> -o <file name>

    does that look right?

    Thanks I am very new to this all!

Latest Articles

Collapse

  • seqadmin
    Investigating the Gut Microbiome Through Diet and Spatial Biology
    by seqadmin




    The human gut contains trillions of microorganisms that impact digestion, immune functions, and overall health1. Despite major breakthroughs, we’re only beginning to understand the full extent of the microbiome’s influence on health and disease. Advances in next-generation sequencing and spatial biology have opened new windows into this complex environment, yet many questions remain. This article highlights two recent studies exploring how diet influences microbial...
    02-24-2025, 06:31 AM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by seqadmin, 03-03-2025, 01:15 PM
0 responses
169 views
0 likes
Last Post seqadmin  
Started by seqadmin, 02-28-2025, 12:58 PM
0 responses
256 views
0 likes
Last Post seqadmin  
Started by seqadmin, 02-24-2025, 02:48 PM
0 responses
636 views
0 likes
Last Post seqadmin  
Started by seqadmin, 02-21-2025, 02:46 PM
0 responses
265 views
0 likes
Last Post seqadmin  
Working...
X