I had this problem too, my solution was to use unix command to trim and fold the fasta file. You would have to cut the header first, and catenate it with your sorted fasta file. It perfectly solves the problem.
Writing a shell script may be a good idea to make things easlier.
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This bioperl snippet fixes the fasta:
Code:use Bio::SeqIO; $in = Bio::SeqIO->new(-file => "inputfilename", -format => 'Fasta'); $out = Bio::SeqIO->new(-file => ">outputfilename", -format => 'Fasta'); while ( my $seq = $in->next_seq() ) {$out->write_seq($seq); }
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I am having this same issue. While I have verified that there are lines of different length in the sequence file, however, why should this matter?
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Originally posted by baohua100 View Postpopulus@Rust:~/samtools-0.1.5c_x86_64-linux$ ./samtools faidx /media/Poplar/baohua/genome/poplar_genome.fa
[fai_build_core] different line length in sequence 'scaffold_28'.
Segmentation fault
What's the meaning of defferent line length ?
It's the standard fasta file.
I download it from:
ftp://ftp.jgi-psf.org/pub/JGI_data/P...asked.fasta.gz
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samtools faidx Segmentation fault
populus@Rust:~/samtools-0.1.5c_x86_64-linux$ ./samtools faidx /media/Poplar/baohua/genome/poplar_genome.fa
[fai_build_core] different line length in sequence 'scaffold_28'.
Segmentation fault
What's the meaning of defferent line length ?
It's the standard fasta file.
I download it from:
ftp://ftp.jgi-psf.org/pub/JGI_data/P...asked.fasta.gzTags: None
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