Dear All,
I have browsed old forums but I really don't know how to fix problem,
I mapped SOLiD reads with LifeScope, then I run both cufflinks and cuffmerge, now when I run cuffdiff I encounter the XS tag problem, how do i fix it?
any experience?
from cufflinks web site:
However Cufflinks will accept SAM alignments generated by any read mapper. Here's an example of an alignment Cufflinks will accept:
and what about cuffdiff?
thanks,
paolo
samfile:
I have browsed old forums but I really don't know how to fix problem,
I mapped SOLiD reads with LifeScope, then I run both cufflinks and cuffmerge, now when I run cuffdiff I encounter the XS tag problem, how do i fix it?
any experience?
from cufflinks web site:
However Cufflinks will accept SAM alignments generated by any read mapper. Here's an example of an alignment Cufflinks will accept:
and what about cuffdiff?
thanks,
paolo
samfile:
Code:
691_1862_244 16 chr1 3000885 1 5S25M20S * 0 0 CTGCGGGCATTNNGTACCATGCGGNNNGGGTAATAACGGAAATAATTGTA ...367...-+%( CFFJJJJJJ?3*#'+,,52222222221111112444 RG:Z:SHAM_1 NH:i:1 CM:i:2 NM:i:0 CQ:Z:/?;//@@@//;@;6666@@/?>6/22//2@@2/<2@@/26@@?@=@62@@ CS:Z:T031103033002031 03303100010003313101310110313003312 426_2037_1220 16 chr1 3034383 1 5S25M3S17H * 0 0 GACAGTCTTTTAAGCCCGTGGTTGTGTCTNCGN //4559:::9088GAJJJJJJJJJJHFF2 )1+' RG:Z:SHAM_1 NH:i:1 CM:i:2 NM:i:3 CQ:Z:/@@@22@/88/@<8@/@//2@//@2/6/@=6@/6>6>/@;/@;@28@=2< CS:Z:T32223212221022230133202211110101130032030002212 112 670_1721_1819 0 chr1 3063656 5 20S27M3S * 0 0 CCCCCCACTTTGAGGGTTTCACAAACAGAGGCATAAGGNNNNAGTGTCNN >644444444444 6888<AE>>DJJJJJJJJJJJJ:90$&$&+577?-*) RG:Z:SHAM_1 NH:i:1 CM:i:2 NM:i:0 CQ:Z:6//@=6@/@=6?@/2@@26;@@//6=@/@@@2@8?/@/*/</2.2@@//2 CS:Z:T200000112001220 01002111001122203133020200232111200