Hi guys,

Can anyone help explain 1) how p value is calculated for the differential expression using cuffdiff for two conditions without replicates? 2) is this p value useful or we can just look at the fold change to select DE genes?

It is easy to understand p values with replicates, but doesn't make much sense to me to use p or q values for 2 samples without replicates.

Many thanks!

Can anyone help explain 1) how p value is calculated for the differential expression using cuffdiff for two conditions without replicates? 2) is this p value useful or we can just look at the fold change to select DE genes?

It is easy to understand p values with replicates, but doesn't make much sense to me to use p or q values for 2 samples without replicates.

Many thanks!

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