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  • How to visualize mapped RNAseqs and annotations?

    I have a bunch of supercontigs with RNAseqs mapped to them in an ace file. In a gtf file I have the annotations for these supercontigs. How can I display the contigs with the mapped reads and the annotations at the same time?
    I am currently using Geneious Pro.

    Thanks!

    Jon
    Last edited by JonB; 09-24-2012, 11:45 PM. Reason: Spelling mistake

  • #2
    Re: How to visualize mapped RNAseqs and annotations?

    Use any genome visualizer such as the IGV - Integrative Genomics Viewer.
    Its stand-alone and quite simple.

    But if you're gonna try and make manipulations later, NCBI genomics workbench is a better go.


    Regards,
    Rohit
    Last edited by rohitngs; 09-24-2012, 11:57 PM.

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    • #3
      visualization

      GBrowse2 is my flavor of choice.

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      • #4
        Hi Jon,

        try Tablet (http://bioinf.scri.ac.uk/tablet/). It takes ace files and you can import annotation as tracks in GFF format (if you have annotation in your GTF file you may need to tweak this slightly but as far as I know the rest of the file format is identical between the two).

        If your ace file is really big you may want to consider BAM instead, if that's an option -- there are no size contraints with that format.

        cheers

        Micha

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        • #5
          Thanks!
          I tried Tablet and it works fine. Only had to increase the memory in order to import the ace file.

          Comment


          • #6
            Hi there,

            you could also use Integrated Genome Browser

            Comment

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