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  • Assessing RNA degradation in RNA-Seq

    I've seen 3'-5' coverage plots used to diagnose RNA degradation for RNA-Seq experiments. Before I begin writing my own I was just wondering if anyone knows of an existing script that can produce them (from a BAM and a GTF for instance)?

  • #3
    Nice, thanks!
    I was also interested in some easy way to get what the geneBody_coverage.py module is plotting.

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    • #4
      Hi, have you tried ngs.plot? It is a very powerful and flexible tool to plot NGS samples. In addition to a global plot for the entire genome, you can create any combination of bam files and gene lists. You can plot RNA-seq or ChIP-seq which are implemented as two different modes.

      As for your question, click this example .
      Last edited by asiangg; 06-19-2013, 09:42 AM.

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